アブストラクト（56巻2号：神奈川歯学）

神奈川歯学

English

Title　： Localization of glial cell marker proteins in the rat mesencephalic trigeminal nucleus
Subtitle　： ORIGINAL ARTICLE
Authors　： Kota WATANABE1), Takeshi OGUCHI2), Sakura ONOUE3), Shingo MAEDA4), Kazuyoshi HIGASHI4), Akira KAWATA4)
Authors（kana）　：
Organization　： 1)Graduate School of Dentistry, Kanagawa Dental University, 2)Department of Anatomy, Kanagawa Dental University, 3)College of Science and Engineering, Kanto Gakuin University, 4)Department of Molecular Oral Histology and Embryology, Kanagawa Dental University
Journal　： Kanagawa Shigaku
Volume　： 56
Number　： 2
Page　： 101-109
Year/Month　： 2021 / 12
Article　： Original article
Publisher　： Kanagawa Odontological Society
Abstract　： [Abstract] Oral dyskinesia, defined as repetitive involuntary movements appearing in the oral and maxillofacial region, is an oral function abnormality that decreases the quality of life of patients. However, the pathogenesis of oral dyskinesia remains unknown and the condition is often difficult to treat. Since 2000, glial cells have attracted attention as a target for therapeutic agents for Parkinson's disease, which is characterized by the same involuntary movements as oral dyskinesia. However, the distribution and role of glial cells in brainstem regions such as the pons and the medulla oblongata, where many neurons involved in oral and maxillofacial movements are located, have not been studied in detail. Thus, this study performed immunohistochemical analysis to investigate the localization of glial cell marker proteins in the mesencephalic trigeminal nucleus (Me5), which is involved in the control of jaw movement. To do so, the brainstem regions of rats were removed and coronal sections were prepared, followed by immunofluorescence staining for the glial cell markers glial fibrillary acidic protein (GFAP), S100 protein, and neuron-glial antigen 2 (NG2), which showed a large number of GFAP-, S100-, and NG2-positive cells around neurons in the Me5. These results suggested that glial cell marker-positive cells in the Me5 secrete ATP and other signaling molecules and are involved in jaw reflexes such as the mandibular tensor and periodontal masseter reflexes, which regulate mandibular movements. Future studies will evaluate the expression of these marker molecules during postnatal development and investigate the effects of glial cells on neural circuit formation in the Me5 in detail, to elucidate the pathogenesis of oral dyskinesia and other oral dysfunctions.
Practice　： Dentistry
Keywords　： mesencephalic trigeminal nucleus, GFAP, S100, NG2