アブストラクト(49巻1号:神奈川歯学)

神奈川歯学

Japanese

Title : ヒト歯槽骨骨膜由来細胞スフェロイド内の細胞分化に対する低出力パルス超音波の影響
Subtitle : 原著
Authors : 宗正憲和, 東一善*, 高橋理*, 出口眞二
Authors(kana) :
Organization : 神奈川歯科大学大学院歯周組織再生学講座, *神奈川歯科大学大学院神経組織発生学講座
Journal : 神奈川歯学
Volume : 49
Number : 1
Page : 41-55
Year/Month : 2014 / 6
Article : 原著
Publisher : 神奈川歯科大学学会
Abstract : 「緒言」近年, 歯周組織再生誘導法(guided tissue regeneration : GTR)やエナメルマトリックスタンパク質(enamel matrix derivative : EMD)を応用した方法, 自家骨移植術などが歯周病により失われた歯周組織の再生に用いられ, 成果をあげている. しかし, これらの方法は2度の根分岐部病変や2, 3壁性骨欠損などが適応症であり, 水平性骨欠損と比較し垂直的骨欠損の再生に有意である. そこで, 我々は3次元構造を有するスフェロイドの歯周組織再生療法への応用に着目し, スフェロイド作製に患者の細胞を用いることを想定し, ヒト歯周組織由来細胞を用い研究を重ねてきた. その結果, スフェロイドは細胞外マトリックスを含む多数の細胞の凝集塊であり, 1週間でコンフルエントになる単層培養と比較して, 3次元培養によるスフェロイド内の細胞は数週間, 細胞分化や細胞活性が持続できることを示してきた. その分化能の持続は細胞の立体的な構築が, 主な要因と考えられている.
Practice : 歯科学
Keywords : 歯槽骨骨膜由来細胞, スフェロイド, 低出力パルス超音波

English

Title : Effects of Low-intensity Pulsed Ultrasound on the Differentiation of Human Alveolar Bone Periosteal Cells in Spheroids
Subtitle :
Authors : Norikazu MUNEMASA, Kazuyoshi HIGASHI*, Osamu TAKAHASHI*, Shinji DEGUCHI
Authors(kana) :
Organization : Department of Periodontal Regeneration, Kanagawa Dental University Graduate School, *Department of Neuroanatomy, Histology and Embryology, Kanagawa Dental University Graduate School
Journal : Kanagawa Shigaku
Volume : 49
Number : 1
Page : 41-55
Year/Month : 2014 / 6
Article : Original article
Publisher : Kanagawa Odontological Society
Abstract : [Abstract] We described that low intensity pulsed ultrasound (LIPUS) promoted periodontal tissues healing following periodontal flap surgery in experimental alveolar bone defects, but the exact mechanism is unknown. Spheroids systems are a form of three-dimensional cell culture that promotes cell matrix interaction, which could recapitulate the aspect of cell homeostasis in vivo. Therefore, we evaluated the effects of LIPUS on cell morphology and differentiation of human alveolar bone periosteum-derived cells (HABPCs) in spheroids. HABPCs were suspended at a cell density of 1.8 x 104 cells/cm2 in a 100-mm culture dish. After 10 days cultivation, the cells formed spheroids, which were digested by 0.25% trypsin, and centrifuged at 300 g for 3 min. LIPUS treatment (1.5 MHz, 30 mW/ cm2) was applied to spheroids for 15 min daily for up to 14 days. The effects of LIPUS exposure on HABPCs in spheroids were observed by histological, immunohistochemical, and microscopically techniques. The three-layer structure and funicular structures were observed in the core from outer layer of spheroids after 1 day exposure. Rough endoplasmic reticulum, mitochondria, endosome, and dense granules were observed in the cytoplasm of HABPCs in spheroids with transmission electron microscope after 1 day exposure. The threelayer structure was formed faster in this study compared with non-irradiated spheroids. We observed osteopontin positive reaction products at the all layer and osteocalcin positive reactions at the middle layer and the core of spheroids with immunohistochemical analysis after 7 days exposure. These results suggested that LIPUS promoted differentiation of HABPCs in three-dimensional structure.
Practice : Dentistry
Keywords :