アブストラクト（16巻1号：神奈川歯学）

神奈川歯学

Japanese

Title　： 家兎大腿骨および下顎骨に分布するestrogen結合物質の成長発育に伴う量的変動
Subtitle　： 原著
Authors　： 秋本進
Authors（kana）　：
Organization　： 神奈川歯科大学矯正学教室
Journal　： 神奈川歯学
Volume　： 16
Number　： 1
Page　： 33-48
Year/Month　： 1981 / 6
Article　： 原著
Publisher　： 神奈川歯科大学学会
Abstract　： 「抄録」 骨の成長発育に対する性ステロイドホルモンの影響を知るために, 種々の成長発育段階にある日本白色家兎を用いて, 下顎骨や大腿骨におけるestrogen binding substanceの分布量について, 年齢および性別との関連から検索した. 実験には3, 6, 11あるいは16週齢の雌雄の動物を用い, 下顎骨からは下顎頭部, 骨体部および前歯部歯槽骨を, また大腿骨からは遠位骨端部および骨幹部を分断採取し分析用試料とした. 取り出した骨組織試料は細片したのち細胞破壊を行い, 4℃, 105000×g, 60分間遠心分離し, estrogen receptorを含むcytosol分画を調製した. こうして得たcytosol分画とestradiol〔2, 4, 6, 7-3H〕-17βとをin vitroで結合反応させ, 標識化estrogen binding substance(EBS)を得た. 次に遊離3H-estradiolをactive dextran coated charcoalに吸着させて除去したのち, EBSが骨組織各部に分布する量を測定するとともにそのものの性状を分析した. 各試料を分析したところ以下のような結論を得た. 1. 骨組織細胞にはestradiol-17βの受容機構が存在し, 特に成長発育の旺盛な下顎頭部および大腿骨遠位骨端部の細胞に多量に分布していた. 2. EBSをSephadex G-50でゲル濾過して得た3つの分画(FI, FIIおよびFIII)のうち, FIは特に骨端軟骨の成長発育と強い関連を示し, 急激な成長の開始時期に相当する6週齢で雌雄とも多量に分布した. 3. 骨端軟骨のFIをSephadex G-200でゲル濾過して得た分画(G-200IおよびG-200II)のうち, G-200Iは3週齢時全体の70%以上を占め, しかも雄に多量に分布した. またG-200IIは6週齢時増量して, 全体に占める割合はG-200Iよりも高くなる傾向を示した. 4. これらG-200IおよびG-200IIはそれぞれ分子量6万から20万までの幾つかの成分から構成される多様体であり, その組成には各組織の成長発育段階による差や部位別差, 性差が認められた. 5. 以上のことから骨組織の中でも特に成長の旺盛な骨端軟骨は明らかにestradiol-17βの影響を受けており, 成長スパート開始前に急速に細胞内にreceptorを合成し, ステロイドホルモンと結合する. これがアロステリック効果によって特定遺伝子を活性化して蛋白合成を調節していることが推定される.
Practice　： 歯科学
Keywords　：

English

Title　： The Difference in Distribution of Estrogen Binding Substance in Cytosol of Rabbits' Femur and Mandibula with Growth and Development
Subtitle　：
Authors　： Susumu AKIMOTO
Authors（kana）　：
Organization　： Department of Orthodontics, Kanagawa Dental College
Journal　： Kanagawa Shigaku
Volume　： 16
Number　： 1
Page　： 33-48
Year/Month　： 1981 / 6
Article　： Original article
Publisher　： Kanagawa Odontological Society
Abstract　： For the purpose of studying the effects of sex steroid hormones on bone growth and development, the distribution of receptors bound with hormone were examined in the mandible and the femur of male and female Japanese white rabbits at the age of 3, 6, 11 or 16 weeks. At autopsy, the bone and blood samples were rapidly removed from subjects, and then the mandible was separated into the condyle, the ramus including a part of body (the body) and the alveolar process (the alveolar bone) in anterior portion, and the femur was also divided into the distal extremity (the condyle) and the shaft (the diaphysis) in room at 4℃, respectively. Following disruption of the cells in these bone specimens using homogenizer and applying ultrasound, cytosol fractions were isolated from the bone and blood cells separately with centrifugalization at 4℃, 105000×g for an hour. Those cytosol fractions were then incubated in medium prepared for radioreceptor assay using estradiol [2, 4, 6, 7-3H] as a tracer. After that, free 3H-estradiol were removed with active dextran coated charcoal. The amounts of radioactivity, i. e., the rate of localization of radioactive estrogen binding substances (EBS) in the each portions of bones and serum, were determined in a liquid scintillation counter. Furthermore, qualitative analyses of EBS were performed on Sephadex G-50 or G-200 gel chromatography and polyacrylamido gel SDS electrophoresis. The analytic results of those specimens were as follows ; 1. Receptors bound with 3H-estradiol or EBS appeared in the bone cells. The rate localized in the mandibular and femoral condyles, where an intense and rapid growth and development was observed, were undoubtedly higher than that of the other portions. 2. EBS were separated into three major peaks (FI, FII and FIII) from elution pattern of molecular sieve chromatography on Sephadex G-50. The amounts of FI had a very close reference to growth and development of the cartilage plate in the extremity and increased in male and female at the age of six weeks equivalent for the beginning of an intense and rapid growth and development. 3. Furthermore, FI were divided into two fractions (G-200I and G-200II) on Sephadex G-200 chromatogram. The rate of localization of G-200I in the chondroepiphyseal tissues was over seventy percent at the age of three weeks, and the amounts in male were greater than that of female. On the other hand, the values of G-200II increased at the age of six weeks. The rate of G-200II in F I tended to be higher than that of G-200I. 4. G-200I and G-200II were composed with a few kinds of components which were from sixty to two-hundred thousands molecular weight. Those compositions differed in the bones, growing or developmental stages, and sex. 5. Those results suggest that biosynthesis of receptors in the condroepiphyseal tissue cells would be accelerated at the beginning of growth spurt, and steroid hormone receptor complexes might be able directly to regulate gene transcription and synthetic activity of protein.
Practice　： Dentistry
Keywords　：