アブストラクト（32巻2号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： Matrix metalloproteinase (MMP) production from stromal fibroblast promotes cancer invasion
Subtitle　： Selective Proceedings of 38th General Meeting of Kanagawa Odontological Society, 2003
Authors　： Satoshi Shimizu, Shigeyuki Ozawa, Eiro Kubota
Authors（kana）　：
Organization　： Department of Oral and Maxillofacial Surgery, Kanagawa Dental College
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 32
Number　： 2
Page　： 126-129
Year/Month　： 2004 / 9
Article　： Report
Publisher　： Kanagawa Odontological Society
Abstract　： Cancer cell invasion and metastasis are very complex process that requires the interaction between cancer cell and stromal cell. Our previous study showed that in 3-dimensional (3-D) coculture invasion model using type I collagen matrigel, pro-MMP-9 (92-kDa) derived from an oral squamous cell carcinoma cell line, HSC-3, was converted into fully active-MMP-9 (67-kDa) by MRC-5 fibroblast-derived factors and the cancer cell invaded into the type I collagen gel. To investigate the proteinases responsible for cancer invasion via interaction with fibroblast, kinetics of the appearance of proteinases and cancer cell invasion were simultaneously examined in this study. Cancer cell started invasive growth at the sixth day of culture, and activation of cancer cell-derived MMP-9 was associated with this step by promoting MMP-1 and MMP-3 production. Interestingly, MMP-3 and MMP-1 mRNA from MRC-5 fibroblasts were up-regulated by soluble factors derived from HSC-3. The HSC-3 cell migration into the type I collagen gel was also enhanced by the culture supernatant from the MRC-5 fibroblast. These results suggested that stromal fibroblast was indispensable to cancer cell invasion.
Practice　： Dentistry
Keywords　： MMPs, cancer cell, invasion, stromal fibroblast, 3-D co-culture