アブストラクト（30巻1号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： Effect of Enamel Matrix Derivative on Migration of Human Periodontal Ligament Fibroblasts
Subtitle　： ORIGINAL ARTICLES
Authors　： Shinji Deguchi1, Kazuhiro Tago1, Osamu Yamashita1, Satoru Sato1, Yukiko Koke1, Akira Sugaya1, You Iijima2, Toshio Kawase2,3
Authors（kana）　：
Organization　： 1Department of Periodontology, Kanagawa Dental College, 2Dental Bioengineering, Kanagawa Dental College, 3Institute for Frontier Oral Science, Kanagawa Dental College
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 30
Number　： 1
Page　： 27-31
Year/Month　： 2002 / 3
Article　： Original article
Publisher　： Kanagawa Odontological Society
Abstract　： The migrational behavior of fibroblasts is critical for the maintenance and healing of the periodontium. Our previous studies showed that the conditioned-medium derived from human gingival fibroblasts (HGFs-CM) enhanced the chemotactic activity of human periodontal ligament fibroblasts (HPLFs). Embryonic enamel matrix proteins are hypothesized to be involved in the formation of acellular cementum during tooth development, suggesting that these proteins can be used to regenerate periodontal tissues. This study examined whether there was an effect of migration of HPLFs to an enamel matrix derivative (EMD). HGFs were cultured to days 3, 5, and 7 in Dulbecco's modified Eagle medium (D-MEM). The conditioned-media were collected as EMD-HGFs-CMs after 24 hours incubation in the presence of varying concentrations of EMD (100, 10, and 1 μg/ml) in serum-free medium (MCDB 107), and the cell numbers were determined. A chemotactic assay of HPLFs was carried out in a modified 96-well Boyden chamber apparatus consisting of an upper and lower compartment separated by an 8-μm pore polycarbonate framed filter (standard). HPLFs (225μl, 2.5×10 5 cells/ml) were placed in the upper compartment. Three different concentrations of EMD (100, 10, and 1μg/ml), EMD-HGFs-CMs and HGFs-CM (positive control) were used as chemoattractants in the lower compartments. The chamber was incubated at 37℃ in a humidified atmosphere of 5% CO2 for six hours. The several concentrations of EMD and 100μg/ml of EMD-HGFs-CM did not stimulate the migratory activity of HPLFs, however 10 and 1μg/ml of EMD-HGFs-CM enhanced (p<0.05) the migration activity for HPLFs at days 3 and 5 compared with HGFs-CM. These findings suggest that EMD might act as a matrix for the migration of fibroblasts with HGFs-CM. The enamel matrix derivative may play an important role with HGFs in periodontal tissue remodeling, and consequently, be useful for clinical application in periodontal regeneration.
Practice　： Dentistry
Keywords　： Migration, Periodontal regeneration, Enamel matrix, Fibroblast