アブストラクト（20巻1号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： Significance of Submandibular Gland for the Aromatase Activity in Ovary of Rat
Subtitle　： ORIGINAL ARTICLES
Authors　： Shouhei Kogure, Mayumi Tarui, Shinri Koshika
Authors（kana）　：
Organization　： Department of Biochemistry, Kanagawa Dental College
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 20
Number　： 1
Page　： 3-8
Year/Month　： 1992 / 3
Article　： Original article
Publisher　： Kanagawa Odontological Society
Abstract　： [Abstract] To investigate the physiological action of the rat submandibular gland (SMG) on the reproductive function, the aromatase activities were assayed in the ovary of adult rats after the extirpation of SMG at 6 weeks of age. The ovaries were obtained 1, 4 and 18 weeks after the operation. The amount of 3H2O, formed during the conversion of 1β-3H-Δ4-androstenedione, resulting from the release during aromatization, was measured for the estimation of aromatase activities. The Vmax of the enzyme activities was clearly enhanced by the operation, showing a constant Km value for the substrate. The enhanced ovarian aromatase activities of adult rats were revealed to have decreased by the SMG extract. On the contrary, the enzyme activities in the ovary of a rat sialoadenectomized at an infant stage were raised by the adult SMG extract in a dose-dependent fashion. The concentration of estradiol-17β in plasma increased by the ectomy, however, LH and FSH were not affected. It is concluded that adult female rat SMG contains the factor(s) which has a suppressive effect on the aromatase activities in the ovarian microsome and that the binding protein to the factor is not formed in the ovary at the infant stage.
Practice　： Dentistry
Keywords　： Aromatase, Ovary, Submandibular Gland